我们建立的离休豚鼠肺灌流装置,可以连续描记肺重量和肺动脉压的变化。在此模型上研究了磷脂酶A_2(PLA_2)对肺的损伤作用。获得的结果是:灌流液加PLA_2(46U/ml)后,30min内肺重量进行性增加,肺动脉压无明显变化。30min时肺白蛋白通透性表面乘积、肺表面渗出液、肺水含量、肺系数和灌流液中血管紧张素转换酶(ACE)活性明显增加。PLA_2抑制剂对溴苯酚基溴可阻断上述变化。白三 We have established a recuperation guinea pig perfused device that continuously characterizes changes in lung weight and pulmonary pressure. In this model, the effect of phospholipase A2 (PLA2) on lung injury was studied. The results obtained are: pulmonary perfusion fluid plus PLA_2 (46U / ml), within 30min progressive increase in lung weight, no significant change in pulmonary arterial pressure. Pulmonary albumin permeability surface product, pulmonary surface exudate, lung water content, pulmonary coefficient and angiotensin converting enzyme (ACE) activity in perfusate significantly increased at 30 min. PLA_2 inhibitor bromophenol bromine can block these changes. White three