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黄杨是栽植很广的庭园观赏灌木,美国长木植物园从世界各地收集了许多优良品种.仅从形态上来区别这些黄杨品种是很困难的.为鉴定20个黄杨优良品种,我们分别从形态特征(叶、习性和长势)和分子标记(AFLP标记)两方面着手,综合分析它们的亲缘关系.三个AFLP引物结合在75和500bp DNA片段间一共产生212个条带.每一个黄杨品种平均有74个条带,最低者有50条,最高者有108条;通常可用于签定的特有条带在0到15条之间.品种间的遗传距离介于0.028和0.439之间.用PAUP软件,我们得到一个UPGMA树状图,并将每一步有显著差异的标在图上.从分子特性来讲,这20个黄杨优良品种可能起源于4到5个种,并可很清楚地分为12个栽培变种.形态特征也支持分子特性的结论.由此可见,结合形态特征和分子标记是确定遗传关系、鉴定栽培变种和分子辅助定性育种的一个很好的方法.
Boxwood is a very ornamental garden ornamental shrubs, the United States Longwood Botanical Garden collected from around the world many good varieties only from the morphological distinction of these Boxer varieties is very difficult to identify 20 good varieties of Boxwood, respectively, we from the morphological characteristics Leaf, habit and growth) and molecular marker (AFLP marker), we analyzed the relationship between AFLP primer and AFLP primer.The three AFLP primers combined to produce 212 bands between 75 and 500 bp DNA fragments.The average number of each boxwood species was 74 With the lowest of 50, the highest of 108. The unique bands usually available for signature are between 0 and 15. The genetic distance between cultivars is between 0.028 and 0.439.Using PAUP software, We obtained a UPGMA dendrogram and marked each step with significant differences.From the molecular characteristics, the 20 elite Populus species may have originated from 4 to 5 species, and can be clearly divided into 12 Morphological characteristics also support the conclusions of the molecular characteristics.Thus, the combination of morphological characteristics and molecular markers is to determine the genetic relationship, identification of cultivars and molecular-assisted qualitative breeding is a good way.