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目的：研究四氢巴马汀（ＴＨＰ）同类物对福尔马林致痛诱导的Ｆｏｓ蛋白表达的影响，以阐明ＴＨＰ同类物的镇痛机制．方法：在右后肢脚掌皮下注射５％福尔马林５０μＬ，诱发炎性疼痛，用免疫组织化学方法观察Ｆｏｓ蛋白表达．结果：腹腔注射ＴＨＰ同类物和Ｄ２受体拮抗剂螺哌隆诱导的Ｆｏｓ蛋白表达主要位于纹状体和伏膈核．Ｄ２受体激动剂喹吡罗可阻滞ｌＴＨＰ和螺哌隆诱导的Ｆｏｓ蛋白表达．ＴＨＰ同类物明显增加脑干下行痛觉调制系统的Ｆｏｓ蛋白表达，并能明显抑制福尔马林诱导的脊髓背角浅层和深层的Ｆｏｓ蛋白表达．结论：ＴＨＰ同类物通过阻滞纹状体和伏膈核的Ｄ２受体，加强脑干下行痛觉调制系统的功能，抑制外周痛觉信息在脊髓水平的传入，达到它们的镇痛作用．
AIM: To investigate the effect of tetrahydropalmatine (THP) congeners on the Fos protein expression induced by formalin in order to elucidate the analgesic mechanism of THP congeners. Methods: The right hind paw subcutaneous injection of 5% formalin 50μL, induced inflammatory pain, immunohistochemistry Fos protein expression was observed. Results: Fos protein expression induced by intraperitoneal injection of THP congeners and D2 receptor antagonist spiperone was mainly located in the striatum and phrenic nucleus. D2 receptor agonist quinopril can block l THP and spiperone induced Fos protein expression. THP congeners significantly increased the expression of Fos protein in the down-regulation system of the brainstem, and significantly inhibited the expression of Fos in the superficial and deep spinal dorsal horn induced by formalin. CONCLUSIONS: THP congeners, through blocking the D2 receptors of the striatum and phrenic nucleus, enhance the function of the down-regulating pain-regulating system in the brainstem and inhibit the introduction of peripheral pain information at the spinal cord level to achieve their analgesic effect.