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目的：观察葛根素在减轻豚鼠卡那霉素耳中毒中的作用。方法：豚鼠３０只随机分为两组：对照组１５只，肌注卡那霉素４００ｍｇ／（ｋｇ·ｄ），共１２ｄ；葛根素组１５只，除肌注卡那霉素４００ｍｇ（ｋｇ／ｄ）外，腹腔注射葛根素１０ｍｇ／（ｋｇ·ｄ），共１２ｄ。比较耳郭反射、内耳生物电及听觉诱发电位。结果：（１）耳郭反射阈（ＰＲ）变化：对照组２６耳２、４、８ｋＨｚ ３个频率听力均下降，异常率为８７％；葛根素组仅６耳于上述３个频率听力下降，异常率仅为２０％；两者差异有高度显著性（Ｐ＜０．０１）；（２）内耳生物电：０、８０ｄＢ短声刺激引起的耳蜗微音器电位（ＣＭ）与听神经电位（Ｎ１）的幅值葛根素组明显高于对照组（Ｐ＜０．０５）；（３）听觉诱发电位（ＢＡＥＰｓ）：ＢＡＥＰｓ阈值对照组明显高于葛根素组（Ｐ＜０．０５）；各波幅值则明显低于葛根素组（Ｐ＜０．０１）。结论：葛根素能减轻卡那霉素耳毒性，对卡那霉素耳中素具有一定的保护作用，为耳毒性抗生素致聋的防治提供了新的研究途径。
Objective: To observe the effect of puerarin in reducing kanamycin ototoxicity in guinea pigs. Methods: Thirty guinea pigs were randomly divided into two groups: control group (n = 15), intramuscular injection of kanamycin (400mg / (kg · d)) for 12 days; d), intraperitoneal injection of puerarin 10mg / (kg · d) for 12 days. Compare auricle reflex, inner ear bioelectric and auditory evoked potentials. Results: (1) The changes of auricular reflex threshold (PR): The hearing loss of 3 ears at 2, 4, 8 and 8 kHz of the control group all decreased, with an abnormal rate of 87%; only 6 ears of puerarin group had decreased hearing at the above 3 frequencies, The abnormal rate was only 20%. The difference between the two groups was highly significant (P <0.01). (2) The bioelectricity of the inner ear: 0,80dB short-tone stimulation caused the cochlear microphones potential (P <0.05). (3) BAEPs: The threshold of BAEPs in the control group was significantly higher than that in the puerarin group (P <0.05); the amplitude of the wave puerarin group was significantly higher than that of the control group The amplitude was significantly lower than the puerarin group (P <0.01). CONCLUSION: Puerarin can reduce kanamycin ototoxicity and protect kanamycin from euphorin, which provides a new approach for prevention and treatment of ototoxicity induced deafness.