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目的探讨六价铬(Cr(VI))对L-02肝细胞线粒体能量代谢的影响。方法用浓度为2、4、8、16、32、64、128、256、512μmol/LCr(VI)溶液作用于L-02肝细胞培养12h,以MTT法检测细胞存活指数以及线粒体中琥珀酸脱氢酶的活性;选取细胞存活率大于70%的5个Cr(VI)浓度,用高效液相色谱法检测不同浓度Cr(VI)对肝细胞中腺苷酸(ATP,ADP,AMP)含量的影响,并分析ATP/ADP比值以及负荷能量(EC)的变化。结果在2~512μmol/LCr(VI)处理浓度,Cr(VI)对L-02肝细胞具有明显的细胞毒性,其细胞存活指数与Cr(VI)处理浓度呈明显负相关(r=-0.933);在4~32μmol/LCr(VI)浓度范围,染毒组肝细胞内腺苷酸(ATP,ADP,AMP)含量以及ATP/ADP比值随Cr(VI)染毒浓度的增加呈明显下降趋势,与对照组比较P<0.05,而EC无明显变化(P>0.05)。结论Cr(VI)对L-02肝细胞线粒体能量代谢存在明显抑制作用。
Objective To investigate the effect of Cr (VI) on mitochondrial energy metabolism in L-02 hepatocytes. Methods L-02 hepatocytes were cultured in the concentrations of 2,4,8,16,32,64,128,256,512μmol / L (VI) for 12h. The cell survival index and mitochondrial succinate The activities of Cr (VI) in hepatocytes were detected by high performance liquid chromatography (HPLC). The concentrations of adenosine monophosphate (ATP, ADP, AMP) Influence, and analyze ATP / ADP ratio and load energy (EC) changes. Results Cr (VI) had obvious cytotoxicity on L-02 hepatocytes at the concentration of 2 ~ 512μmol / L (VI), and the cell survival index was negatively correlated with the concentration of Cr (ATP, ADP, AMP) and ATP / ADP ratio in the hepatocytes decreased significantly with the increase of Cr (VI) concentration in the range of 4 ~ 32μmol / Compared with the control group, P <0.05, while EC had no significant change (P> 0.05). Conclusion Cr (VI) has a significant inhibitory effect on the energy metabolism of mitochondria in L-02 hepatocytes.