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目的观察６种已知抗生育活性化合物和２２种可能有抗生育活性的天然化合物对体外培养人绒毛滋养层组织生长晕细胞（以下简称滋养层细胞）和蜕膜细胞的损伤作用，以验证抗生育新药筛选模型并筛选新的抗生育化合物。方法运用人滋养层细胞和蜕膜细胞无血清培养技术，结合ｈＣＧ放免测定，观测筛选微量化合物的抗生育活性。结果已知抗生育化合物Ｒｕ４８６（米非司酮，１００μｇ／ｍｌ）、Ｔａｍｏｘｉｆｅｎ（２０μｇ／ｍｌ）、Ａｎｏｒｄｒｉｎ（１００μｇ／ｍｌ）和Ｔｒｉｃｈｏｔｈａｎｔｈｉｎ（５０μｇ／ｍｌ）对于滋养层细胞的形态和ｈＣＧ分泌功能均有损伤和抑制作用，ＰｓｅｕｄｏｌａｒｉｃａｃｉｄＢ（１００μｇ／ｍｌ）和ＹｕａｎｈｕａｃｉｎｅＡ（０．５μｇ／ｍｌ）主要损伤滋养层细胞的形态。２２种待筛选天然化合物中，１００μｇ／ｍｌ的Ｈｍｌ、Ｈｍｌｏ、ＴＲ４、Ｔ２既损伤人滋养层细胞的形态又抑制其ｈＣＧ的分泌功能。抗生育化合物Ｒｕ４８６（１０μｇ／ｍｌ）、Ｔａｍｏｘｉｆｅｎ（１０μｇ／ｍｌ）、Ａｎｏｒｄｒｉｎ（６０μｇ／ｍｌ）、ＰｓｅｕｄｏｌａｒｉｃａｃｉｄＢ（５０μｇ／ｍｌ）和ＹｕａｎｈｕａｃｉｎｅＡ（０．５μｇ／ｍｌ）对蜕膜细胞有明显形态损伤作?
Objective To observe the effects of 6 known anti-fertility compounds and 22 natural compounds that may have anti-fertility activity on the growth of human corpus luteum trophoblast growth cells (hereinafter referred to as trophoblast cells) and decidual cells in vitro, Fertility new drug screening model and screening of new anti-fertility compounds. Methods The human trophoblast cells and decidual cells were cultured in serum - free medium. Combined with hCG radioimmunoassay, the anti - fertility activities of trace compounds were screened and observed. Results Anti-fertility compounds Ru486 (mifepristone, 100μg / ml), Tamoxifen (20μg / ml), Anordrin (100μg / ml) and Trichothanthin (50μg / ml) were known to have both trophoblast morphology and hCG secretion Injury and inhibition, PseudolaricacidB (100μg / ml) and YuanhuacineA (0.5μg / ml) Main damage trophoblast morphology. Among the 22 kinds of natural compounds to be screened, Hml, Hmlo, TR4 and T2 at 100 μg / ml both damaged the morphology of human trophoblast cells and inhibited the secretion function of hCG. Anti-fertility compounds Ru486 (10μg / ml), Tamoxifen (10μg / ml), Anordrin (60μg / ml), PseudolaricacidB (50μg / ml) and YuanhuacineA (0.5μg / ml)