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螺旋藻培养中常用的Zarrouk氏培养基使用了高浓度的硝酸钠(2.5克/升)。硝酸钠价格昂贵,而且不是我国氮肥工业的主要产品,市场上难以买到,这给降低螺旋藻的生产成本带来了一定的困难。已有的许多试验表明,除了硝态氮外,螺旋藻还能吸收利用铵态氮、酰胺态氮等多种形态氮,其中廉价化肥硫铵和尿素的使用引起了人们的广泛重视。许多配方中用其替代硝酸钠,但报导的用量各不相同。本文从硫铵、尿素的最适用量、抑制浓度、接种后的滞留适应期,光休克反应以及对螺旋藻产量的影响等方面探讨硫铵、尿素的使用。
Zarrouk’s medium, which is commonly used in spirulina culture, uses high concentrations of sodium nitrate (2.5 g / l). Sodium nitrate is expensive and not the main product of China’s nitrogenous fertilizer industry. It is difficult to buy on the market, which brings some difficulties in reducing the production cost of spirulina. Many experiments have shown that in addition to nitrate nitrogen, Spirulina can absorb the use of ammonium nitrogen, amido nitrogen and other forms of nitrogen, of which cheap fertilizer ammonium sulfate and the use of urea has attracted widespread attention. Sodium nitrate is used instead of sodium nitrate in many formulations, but the amounts reported vary. This article discusses the use of ammonium sulfate and urea in terms of the optimum dosage of ammonium sulfate and urea, the inhibitory concentration, the residence time after inoculation, the light shock reaction and the effect on the production of spirulina.