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目的:了解职业苯接触及慢性苯中毒工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达情况。方法:利用SYBR Green I实时荧光定量PCR分别检测20例正常人、25例职业苯接触工人和27例慢性苯中毒工人外周血单个核细胞T-bet和GATA-3 mRNA表达情况。结果:在职业接触苯工人组及慢性苯中毒工人组,多数样本表现为GATA-3表达上升和T-bet表达下降,而少数病人则表现为相反的模式。GATA-3在正常人的表达水平为0.39±0.22,与正常组对照比较职业接触苯工人组中有19例GATA-3表达水平呈上升趋势(0.57±0.54),慢性苯中毒工人组中则有20例GATA-3表达水平呈上升趋势(0.52±0.50)。此外,在职业接触苯工人组中发现6例GATA-3表达水平显著下降(0.15±0.12,P<0.05),同样在慢性苯中毒工人组中也有7例GATA-3表达水平显著下降(0.07±0.06,P<0.05)。T-bet在正常人的表达水平为2.15±1.45,与正常组对照比较职业接触苯工人组中有19例T-bet表达水平呈下降趋势(1.91±1.49),慢性苯中毒工人组中T-bet表达水平均呈下降趋势(1.52±0.56)。此外,在职业接触苯工人组中也可发现6例T-bet表达水平显著上升(3.19±2.10,P<0.05)。结论:职业苯接触及慢性苯中毒影响工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达水平。
Objective: To understand the expression of T cell-specific transcription factor T-bet and GATA-3 mRNA in peripheral blood of workers exposed to benzene and chronic benzene poisoning. Methods: The expression of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells of 20 healthy subjects, 25 benzene exposed workers and 27 chronic benzene poisoning workers were detected by SYBR Green I real-time quantitative PCR. RESULTS: In the workers exposed to benzene and chronic benzene poisoning, the majority of samples showed a rise in GATA-3 expression and a decrease in T-bet expression, whereas the minority patients showed the opposite pattern. The expression level of GATA-3 in normal people was 0.39 ± 0.22, compared with the normal control group, the expression of GATA-3 in 19 exposed workers increased by 0.57 ± 0.54, while in chronic benzene poisoning group The expression of GATA-3 in 20 patients showed an upward trend (0.52 ± 0.50). In addition, GATA-3 expression was significantly decreased in 6 workers exposed to benzene (0.15 ± 0.12, P <0.05), and 7 patients in the chronic benzene poisoning group also showed a significant decrease in GATA-3 expression (0.07 ± 0.06, P <0.05). The expression level of T-bet in normal people was 2.15 ± 1.45, compared with the normal control group, the expression of T-bet in 19 exposed workers showed a decreasing trend (1.91 ± 1.49). In the group of chronic benzene poisoning workers, T- bet expression levels showed a downward trend (1.52 ± 0.56). In addition, T-bet expression was significantly increased in 6 of the workers exposed to benzene (3.19 ± 2.10, P <0.05). Conclusion: Occupational benzene exposure and chronic benzene poisoning affect the expression of T cell-specific transcription factors T-bet and GATA-3 in peripheral blood of workers.