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目的探讨二氧化硫(SO2)预处理对心肌缺血再灌注损伤大鼠的保护作用及对心肌组织中SO2/天门冬氨酸氨基转移酶(AAT)体系的影响。方法 24只健康清洁级雄性Wistar大鼠随机分为假手术组、缺血再灌注组(I/R组)、SO2预处理组(I/R+S组)。结扎大鼠左侧冠状动脉30min,再灌注120min,制备心肌缺血再灌注模型;I/R+S组大鼠在缺血前10min用1μmoL.kg-1SO2颈外静脉注射预处理。采用全自动生化检测仪检测大鼠血浆CK和LDH水平,用高效液相色谱法测定其心肌组织中SO2水平,采用蛋白免疫印迹法检测其心肌组织AAT1和AAT2蛋白表达水平。结果缺血再灌注后,I/R组及IR+S组血浆CK、LDH水平均较假手术组明显增高(P<0.01,0.05),I/R+S组血浆CK、LDH水平较I/R组均明显降低(Pa<0.05)。与假手术组比较,I/R组大鼠心肌组织匀浆中SO2水平显著降低(P<0.05),I/R+S组无明显变化;I/R+S组SO2水平较I/R组显著增高(P<0.01)。I/R组大鼠心肌组织AAT1蛋白表达较假手术组显著降低(P<0.01),I/R+S组与假手术组比较差异无统计学意义(P>0.05),而I/R+S组AAT1蛋白表达则较I/R组显著增高(P<0.01);AAT2蛋白表达在3组间的差异无统计学意义(P>0.05)。结论 SO2预处理可明显降低心肌缺血再灌注大鼠血浆中心肌酶活性,具有心肌保护作用,并对心肌组织中SO/AAT体系具有上调作用。
Objective To investigate the protective effect of sulfur dioxide (SO2) preconditioning on myocardial ischemia-reperfusion injury in rats and its effect on myocardial tissue SO2 / aspartate aminotransferase (AAT) system. Methods Twenty-four healthy male Wistar rats were randomly divided into sham operation group, ischemia reperfusion group (I / R group) and SO2 preconditioning group (I / R + S group). The left coronary artery of rats were ligated for 30 minutes and reperfused for 120 minutes to prepare myocardial ischemia-reperfusion model. Rats in I / R + S group were pretreated with 1μmoL · kg-1SO2 external jugular vein 10 minutes before ischemia. The levels of plasma CK and LDH were measured by automatic biochemical analyzer. The levels of SO2 in myocardial tissue were determined by high performance liquid chromatography (HPLC). The protein expression of AAT1 and AAT2 in myocardium was detected by Western blotting. Results After ischemia / reperfusion, the levels of plasma CK and LDH in I / R group and IR + S group were significantly higher than those in sham operation group (P <0.01, 0.05). The levels of plasma CK and LDH in I / R + S group were significantly higher than those in I / R group were significantly lower (Pa <0.05). The levels of SO2 in I / R group were significantly lower than those in I / R group (P <0.05), and there was no significant change in I / R + S group. Compared with I / R group, Significantly higher (P <0.01). The level of AAT1 protein expression in I / R group was significantly lower than that in sham operation group (P <0.01), while there was no significant difference between I / R + S group and sham operation group (P> 0.05) The protein expression of AAT1 in S group was significantly higher than that in I / R group (P <0.01). There was no significant difference in AAT2 protein expression between the three groups (P> 0.05). Conclusion SO2 preconditioning can significantly decrease the activity of myocardial enzymes in myocardium during myocardial ischemia / reperfusion, and has a protective effect on myocardium and up-regulates SO / AAT in myocardial tissue.