论文部分内容阅读
目的:研究大鼠肝中去甲肾上腺素、加压素、ATP的生理浓度.方法:肝细胞通过胶原水解酶灌流肝脏得到.分离的肝细胞加载钙离子荧光指示探针Fura-2,通过显微荧光术测量胞内钙浓度的变化,以荧光比例(F_(340)/F_(280))表示,分别检测不同浓度范围的去甲肾上腺素、加压素、ATP对单个肝细胞胞浆钙离子浓度升高的作用.结果:去甲肾上腺素、加压素、ATP以剂量依赖的方式诱导胞内钙离子浓度的增加.较低浓度刺激,胞内钙浓度呈振荡性增加;随着刺激物浓度的增加,更多的细胞胞内钙离子浓度呈现缓慢或高台样增加 去甲肾上腺素、加压素、ATP诱发钙振荡的最佳浓度分别是100—500nmol/L、50-100pmol/L和<1μmol/L.结论:在大鼠肝脏中,去甲肾上腺素、加压素、ATP的生理性浓度分别在100-500nmol/L、50—100pmol/L和<1μmol/L范围.
Objective: To study the physiological concentrations of norepinephrine, vasopressin and ATP in rat liver.Methods: Hepatocytes were perfused by collagenase into liver, and the isolated hepatocytes were loaded with Fura-2, The change of intracellular calcium concentration was measured by microfluorescence. The fluorescence intensity (F_ (340) / F_ (280)) was used to measure the change of intracellular calcium concentration in a single hepatocyte with different concentrations of norepinephrine, vasopressin and ATP Ion concentration increased.Results: Norepinephrine, vasopressin and ATP induced the increase of intracellular calcium concentration in a dose-dependent manner.With lower concentration stimulation, the intracellular calcium concentration increased oscillatingly.With the stimulation The increase of concentration, more intracellular calcium concentration showed slow or high profile increase norepinephrine, vasopressin, ATP-induced calcium oscillations were the best concentration of 100-500nmol / L, 50-100pmol / L And <1μmol / L.Conclusion: The physiological concentrations of norepinephrine, vasopressin and ATP in rat livers range from 100-500nmol / L, 50-100pmol / L and <1μmol / L, respectively.