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利用Suzuki偶联反应合成了疏水性寡聚芴分子OF,并对其在氯仿溶液中的紫外吸收和荧光光谱进行了表征,表明其具有较大的摩尔吸光系数(1.08×105mol-1 L cm-1)和高荧光量子产率(96%).OF分子分散到水中可形成纳米粒子,动态光散射实验表明其粒径大小约为230 nm.该纳米粒子在水相中仍保持了较大的摩尔吸光系数以及高的荧光量子产率.我们利用MTT的方法对OF纳米粒子对人肺癌A549细胞的毒性进行了测试,结果表明其具有低的细胞毒性,因此可以用于细胞成像.共聚焦激光扫描显微镜成像结果显示OF纳米粒子主要分布在细胞质中,特别是在近核区域周围.与溶酶体染料Lyso Tracker Red共定位结果表明OF纳米粒子主要存在于溶酶体中,因此可以用于对溶酶体的特异性荧光成像.
The hydrophobic oligofluorene, OF, was synthesized by Suzuki coupling reaction. The UV absorption and fluorescence spectra of the hydrophobic oligofluorene were characterized. The results showed that the copolymer has a large molar absorption coefficient (1.08 × 105mol-1 L cm- 1) and high fluorescence quantum yield (96%). OF molecules dispersed into water to form nano-particles, dynamic light scattering experiments showed that the size of the particle size of about 230 nm. The nano-particles in the water phase still maintained a larger Molar extinction coefficient and high fluorescence quantum yield.We tested the toxicity of OF nanoparticles on human lung cancer A549 cells by MTT assay and showed that it has low cytotoxicity and therefore can be used for cell imaging.Confocal laser Scanning microscopy imaging results showed that OF nanoparticles were mainly located in the cytoplasm, especially around the near nuclear region. Co-localization with lysosomal dye Lyso Tracker Red showed that OF nanoparticles mainly exist in lysosomes, Lysosome-specific fluorescence imaging.