论文部分内容阅读
在自制的８株烯醇化酶（ＮＳＥ）ＭｃＡｂ中，经方阵排列滴定筛选出无交叉反应且效价较高的２株ＭｃＡｂ，用辣根过氧化物酶（ＨＲＰ）为标记物，建立了ＮＳＥ－ＭｃＡｂ双位点夹心ＥＬＩＳＡ。经直线回归方程、取代试验、阻断试验检验，该标准曲线近似直线关系，可测定标本中ＮＳＥ的范围为５～５０μｇ／Ｌ。对２３例小细胞肺癌（ＳＣＬＣ）（Ⅰ和Ⅱ期４例、Ⅲ期７例、Ⅳ期１２例）．３２例非小细胞肺癌（ＮＳＣＬＣ），２２例肺良性疾病（ＢＰＤ）和３６例健康体检者血清ＮＳＥ进行了测定，ＳＣＬＣ的阳性率为８２．６％（１９／２３），其中Ⅰ和Ⅱ期为５０％（２／４）；Ⅲ期为８５．７％（６／７），Ⅳ期为９１．７％（１１／１２）；ＮＳＣＬＣ的阳性率为３．１％（１／３２），ＢＰＤ阳性率为４．５％（１／２２）。ＳＣＬＣ的显著高于ＮＳＣＬＣ和ＢＰＤ（Ｐ＜０．０１）；ＳＣＬＣ的Ⅳ和Ⅲ期显著高于Ⅰ和Ⅱ期（Ｐ＜０．０１）。２３例ＳＣＬＣ经综合治疗后，完全缓解组和部份缓解组血清ＮＳＥ水平非常显著低于进展组和无改变组（Ｐ＜０．０１）。本文研究结果证明，ＮＳＥＭｃＡｂ双位点夹心ＥＬＩＳＡ具有灵敏、特异和简便快速的特点，对ＳＣＬＣ的血清学诊断有较高的灵敏度和特异性，对ＳＣ?
Two McAbs with no cross-reaction and high titer were screened by square-array titration in homemade 8 enolase (NSE) McAbs. Horseradish peroxidase (HRP) was used as a marker to establish two McAbs. NSE-McAb two-site sandwich ELISA. The linear regression equation, substitution test, block test test, the standard curve is approximately linear, the range of NSE can be determined specimens for the 5 ~ 50μg / L. Twenty-three small cell lung cancer (SCLC) (4 cases in stage I and II, 7 cases in stage III and 12 cases in stage IV). 32 cases of non-small cell lung cancer (NSCLC), 22 cases of benign lung disease (BPD) and 36 cases of healthy subjects were detected by NSE. The positive rate of SCLC was 82.6% (19/23) (2/4) in stage Ⅲ, 85.7% (6/7) in stage Ⅲ and 91.7% (11/12) in stage Ⅳ. The positive rate of NSCLC was 3.1% (1/32) The positive rate of BPD was 4.5% (1/22). SCLC was significantly higher than that of NSCLC and BPD (P <0.01). Stage IV and III of SCLC were significantly higher than those of stage I and II (P <0.01). After 23 cases of SCLC treatment, the levels of serum NSE in the complete remission group and the partial remission group were significantly lower than those in the progression group and non-change group (P <0.01). The results of this study demonstrate that NSEMcAb two-site sandwich ELISA has the characteristics of sensitivity, specificity and simplicity and rapidness, and has high sensitivity and specificity for serological diagnosis of SCLC.