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利用聚合酶链区应（PCR）和序列特异性寡核苷酸（SSO）探针技术对47例经临床及免疫荧光证实的IgA肾病（IgAN）患者HLA-DRB1、DQA1、DQB1等位基因频率进行了检测。结果显示IgAN患者组DR4基因频率明显高于正常人组,相反DQB1*0602基因频率与对照组相比呈显著下降。IgAN患者中蛋白尿组DR4基因频率显著高于对照组,而肉眼血尿组与对照组无显著差异。约 1/4 DR4基因阳性的IgAN病理表现为局灶节段硬化性肾小球肾炎。 IgAN肾衰组DR4阳性的发生率显著高于非肾衰组。由此可见,IgAN中HLA-DR4基因频率而著增高, DR4阳性IgAN临床多表现蛋白尿,易发生肾衰,病理多呈局灶节段硬化型;DQB1*0602等位基因对IgAN可能有一定抵抗性。这些研究结果提示IgAN有免疫遗传的背景。
The frequencies of HLA-DRB1, DQA1 and DQB1 alleles in 47 patients with IgA nephropathy (IgAN) confirmed by clinical and immunofluorescence were analyzed by polymerase chain reaction (PCR) and sequence specific oligonucleotide (SSO) Tested. The results showed that the frequency of DR4 gene in patients with IgAN was significantly higher than that in normal controls. On the contrary, the frequency of DQB1 * 0602 gene was significantly decreased compared with the control group. The frequency of DR4 gene proteinuria in IgAN patients was significantly higher than that in control group, but the gross hematuria group and control group had no significant difference. About 1/4 DR4 gene-positive IgAN pathology showed focal segmental sclerosing glomerulonephritis. The incidence of DR4-positive IgAN renal failure group was significantly higher than non-renal failure group. Thus, the frequency of HLA-DR4 gene in IgAN increased, DR4-positive IgAN clinical manifestations of proteinuria, prone to renal failure, pathological mostly focal segmental sclerosis; DQB1 * 0602 allele may have a certain IgAN Resistant. These findings suggest that IgAN has a background in immune inheritance.