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目的:检测肝细胞癌 Rb 抑癌基因失活的多种机制。方法:采用多聚酶链反应—限制性片段长度多态性技术和 Southern 杂交方法对肝细胞癌 Rb 基因内含子1杂合缺失、Rb 基因结构异常和 Rb 基因5′端 CpG 岛的甲基化状态进行了分析。结果:在24例肝癌中,12例为杂合子,Rb 基因内含子1杂合缺失率为25%。在10例肝癌中未发现 Rb 基因重排现象,但2例分别在4.5kb 和9.8kb、7.5kb 处缺失。15例肝癌只有1例肝透明细胞癌出现 Rb 基因5′端高甲基化。结论:肝细胞癌Rb 基因的主要失活机制可能为缺失,除此之外,DNA 甲基化异常可能参与某些亚类肝癌 Rb 基因的失活。
Objective: To detect multiple mechanisms of inactivation of Rb tumor suppressor gene in hepatocellular carcinoma. METHODS: Polymerase chain reaction-restriction fragment length polymorphism and Southern hybridization were used for the heterozygous loss of intron 1 of Rb gene, structural abnormality of Rb gene, and methylation status of CpG island at the 5′ end of Rb gene. Analyzed. RESULTS: Of the 24 cases of HCC, 12 were heterozygous and the Rb intron 1 heterozygous deletion rate was 25%. No Rb gene rearrangement was found in 10 cases of liver cancer, but 2 cases were missing at 4.5 kb and 9.8 kb and 7.5 kb, respectively. In 15 patients with hepatocellular carcinoma, there was only one case of hepatocellular carcinoma with Rb gene hypermethylation at the 5′ end. Conclusion: The major inactivation mechanism of Rb gene in hepatocellular carcinoma may be absent. In addition, DNA methylation abnormalities may be involved in the inactivation of Rb genes in some subclasses of liver cancer.