大面积烧伤及其他创伤造成皮肤缺损时,创面需要复盖,这一需要促进了对组织培养皮肤的研究。作者等已研究出能够重复的大量培养人类或动物表皮细胞的方法。本文证实,在体外生长的人类细胞可移植至无胸腺裸鼠身上,并继续生长。实验並初步指出,从游离细胞体外培养而成的成张犬表皮细胞可成功地作自体或异体移植。材料和方法杂种犬剃毛、清洗、消毒后取全层厚度皮肤,浸入含抗生素之基本培养 The need for coverage of wounds in the event of extensive skin burns and other traumatic injuries results in a need to promote the study of tissue culture of the skin. The authors have developed a reproducible method of culturing human or animal epidermal cells in large quantities. This article demonstrates that human cells grown in vitro can be transplanted onto athymic nude mice and continue to grow. Experiments and preliminary pointed out that from isolated cells in vitro cultured from canine canine endothelial cells can be successfully autologous or allograft. Materials and Methods The mongrel dogs were shaved, cleaned and disinfected to obtain a full thickness of the skin, immersed in a basic culture containing antibiotics