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目的:建立HPLC-DAD同时测定白花蛇舌草中6个活性黄酮成分(槲皮素-3-O-桑布双糖苷、芦丁、槲皮素-3-O-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-吡喃葡萄糖苷、槲皮素、山柰酚)含量的方法。方法:采用Kromasil-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05%磷酸梯度洗脱,检测波长254 nm,体积流量1 mL·min-1,柱温30℃。结果:6种活性黄酮成分线性、精密度、稳定性、重复性均符合方法学相关要求,加样回收率为99.40%~99.70%。应用所建方法测定了不同产地白花蛇舌草中6个活性黄酮成分的量。结论:所建方法简单、可行、准确,可用于白花蛇舌草中活性黄酮成分的质量控制。
OBJECTIVE: To establish a HPLC-DAD method for the simultaneous determination of 6 active flavonoids in Hedyotis diffusa Willd. (Quercetin-3-O-Sambuluide, Rutin, Quercetin-3-O-β-D-glucopyranose Glycosides, kaempferol-3-O-β-D-glucopyranoside, quercetin, kaempferol). METHODS: Kromasil-C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of gradient elution of acetonitrile and 0.05% phosphoric acid. The detection wavelength was 254 nm and the volume flow rate was 1 mL · min-1. Results: The linearity, precision, stability and reproducibility of the six active flavonoids all met the requirements of methodology. The recoveries of the active flavonoids were 99.40% -99.70%. The established method was used to determine the content of 6 active flavones in Hedyotis diffusa. Conclusion: The method is simple, feasible and accurate and can be used for quality control of active flavonoids in Hedyotis diffusa.