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探讨过敏性哮喘患者和正常人外周血嗜酸性粒细胞 (EOS)表达 CD40的差异。采集 10例过敏性哮喘患者和 8例正常对照者静脉血用于纯化 EOS,以流式细胞义检测 CD40在 EOS表面的表达 ,EOS胞浆中的 CD40蛋白则以铭疫细胞化学方法显示。结果表明 ,哮喘患者 EOS表面存在 CD40表达 ,但 CD40并不表达于正常人EOS的表面。无论是哮喘患者还是正常人 ,其 EOS的胞浆均可检测到 CD40蛋白。人 EOS均能合成和储存CD40 ,CD40分子可以移行到哮喘患者 EOS的表面而不能移行到正常人 EOS的表面。揭示 CD40在哮喘的发病机理中具有某种作用
To investigate the difference of CD40 expression in peripheral blood eosinophils (EOS) between patients with allergic asthma and normal subjects. Venous blood from 10 patients with allergic asthma and 8 normal controls were collected for purification of EOS. Expression of CD40 on EOS surface was detected by flow cytometry. CD40 protein in cytoplasm of EOS was detected by immunocytochemistry. The results show that there is CD40 expression on the surface of EOS in asthmatic patients, but CD40 is not expressed on the surface of normal human EOS. CD40 protein was detected in the cytoplasm of EOS in both asthmatic and normal subjects. Human EOS synthesizes and stores CD40. CD40 molecules migrate to the surface of EOS in asthmatic patients and can not migrate to the surface of normal human EOS. Revealing that CD40 plays a role in the pathogenesis of asthma