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繁殖期从嘉陵江收集性成熟的大鳍■ 和长吻 野生亲鱼,用Linpe方法(即LHRH-A加多巴胺D2受体拮抗剂地欧酮)或传统的LHRH—A加脑垂体的方法进行催产,定时取血样,用放射免疫方法测定催产过程中血清GTH水平的变化,进一步证实鲇形目鱼类GTH的分泌受到下丘脑分泌的促性腺激素释放激素GnRH和多巴胺的双重调节;排卵和产卵也是以血清GTH的急剧升高为先导的,而最终能否排卵还有赖于血清GTH峰是否超过“排卵阈值”。尽管催产后的大鳍 和长吻 雄鱼血清GTH水平也有一个高峰出现,但血清GTH水平升高幅度都大大低于雌鱼,这种现象在硬骨鱼类可能具有普遍性。
Breeding period collected from the Jialing River Jialing River maturity of the big finfish and wild kiss broodstock, with Linpe method (LHRH-A dopamine D2 receptor antagonist ketone) or traditional LHRH-A plus pituitary gland method of oxytocin, The blood samples were taken regularly and the changes of serum GTH level during the process of oxytocin were determined by radioimmunoassay. It was further confirmed that the secretion of GTH by the hypothalamus was regulated by both hypothalamic gonadotropin-releasing hormone GnRH and dopamine. Ovulation and oviposition were also The dramatic rise in serum GTH as the guide, and ultimately whether ovulation depends on whether the serum GTH peak exceeds the “ovulation threshold.” Although there is also a peak in Serum GTH levels after oxytocin feeding, serum GTH levels are significantly higher than those of females, a phenomenon that may be prevalent in teleost fish.