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目的:观察壳聚糖对围绝经期大鼠血清E2、P及ERα的影响。方法:将30只11～12月龄健康雌性Wista大鼠随机分为壳聚糖组、空白对照组和雌激素对照组各10只。壳聚糖组灌胃壳聚糖1 500 mg/kg,雌激素组灌胃补佳乐1.2 mg/kg,对照组灌胃3 ml双蒸水,每天灌胃一次,连续30天后处死大鼠。用酶联免疫吸附试验法测血清中E2和P的含量;免疫组织化学SABC法检测子宫ERα的表达情况。结果:壳聚糖组大鼠血清E2、P的含量及ERα的平均光密度分别为(59.83±17.24)ng/L、(89.72±15.20)ng/L及(0.064±0.004),高于空白对照组的(44.52±7.28)ng/L、(51.23±7.30)ng/L及(0.052±0.005),E2、ERα的平均光密度低于雌激素对照组的(74.00±6.85)ng/L、(0.079±0.004),差异有统计学意义(P<0.05)。结论:壳聚糖可以提高围绝经期大鼠血清E2和P的含量,增加ERα的表达。
Objective: To observe the effects of chitosan on serum E2, P and ERα in perimenopausal rats. Methods: Thirty healthy female Wistar rats of 11-12 months old were randomly divided into chitosan group, blank control group and estrogen control group. The chitosan group was given chitosan 1 500 mg / kg, and the estrogen group was given gavage of 1.2 mg / kg. The control group was given gavage 3 ml of double distilled water once daily for 30 days and then the rats were sacrificed. The contents of E2 and P in serum were detected by enzyme-linked immunosorbent assay (ELISA). The expression of ERα in uterus was detected by immunohistochemical SABC method. Results: The contents of E2 and P and the average optical density of ERα in the chitosan group were (59.83 ± 17.24) ng / L, (89.72 ± 15.20) ng / L and (0.064 ± 0.004) (44.52 ± 7.28) ng / L, (51.23 ± 7.30) ng / L and (0.052 ± 0.005) respectively. The mean optical density of E2 and ERα in the estrogen control group was (74.00 ± 6.85) ng / L, 0.079 ± 0.004), the difference was statistically significant (P <0.05). Conclusion: Chitosan can increase the content of serum E2 and P and increase the expression of ERα in perimenopausal rats.