论文部分内容阅读
1.通过高培养力材料为桥梁亲本与高蛋白、高糖、高配合力等种质杂交创造了一批培养力高于10%的基因型重组材料。从中筛选出50份进行整理、编目并在国家库长期保存。2、对玉米的细胞、组织及种质资源进行低温、超低温及不同保存条件的试验,首次证明带有药壁的花粉胚耐低温保存,并获得了低温保存的纯系用于育种实践。3.通过花粉及幼胚培养建立了四个无性繁殖系,获得了Je6.clone,甜玉米clone201纯系。Je6等三个系已用作Gus基因转移的受体。4.对获得的70份生物工程纯系分期进行了田间套袋和干燥保存,同时对优良纯系进行了配合力测定,双B早×吉818参加了吉林省区域试验,军单91049参加了省预备试验,产量高达12473,7kg/hm2,比四单19增产16.9%,列11份参试材料的第一名。
1. A series of genotype recombination materials with cultivating power higher than 10% were created by crossing high germplasm materials as bridge parents with high protein, high sugar and high combining ability. Screening out 50 of them for sorting, cataloging and long-term preservation in national libraries. The experiments of cryogenic, ultra-low temperature and different storage conditions on the cell, tissue and germplasm resources of maize showed that pollen embryos with medicinal wall were preserved in cryogenic temperature for a first time, and the cryopreserved pure lines were obtained for breeding practice. 3. Four clonal lines were established by pollen and immature embryo culture and obtained Je6. clone, sweet corn clone201 pure line. Je6 and other three lines have been used as Gus gene transfer receptors. 4. 70 bioengineering pure lines were bagged and dried in the field, meanwhile the determination of combining ability was carried out on the elite pure lines. The double B × Q 818 took part in the regional test in Jilin Province, and the SF 91091 participated in the provincial preparation Test, yield up to 12,473,7 kg / hm2, 16.9% increase over the single 19, column 11 of the first test materials.