应用本所建立的微量全血酶联(ELISA)法检测特异性IgM抗体技术,对540例急性下呼吸道感染患儿进行呼吸道合胞病毒(RSV)病原检测,其实验室间检测结果重复性良好(X~2=17.86,P>0.05)。20份用滤纸保存1年的外周血进行RSV—IgM抗体再检测,与初次检测结果比较,证实了其抗体活性稳定。提示该项技术和采集标本的方法十分适宜基层卫生单位在RSV流行病学调查中应用。 We established the trace amount of whole blood enzyme ELISA (ELISA) detection of specific IgM antibody technology, 540 cases of children with acute lower respiratory tract infection of respiratory syncytial virus (RSV) pathogen detection, the inter-laboratory test results were reproducible (X ~ 2 = 17.86, P> 0.05). RSV-IgM antibodies were retested in 20 of the peripheral blood preserved for 1 year by filter paper, which was consistent with the first test results and confirmed their stable antibody activity. The techniques that suggest this technique and the methods of collecting specimens are well suited for use by primary health units in the RSV epidemiological survey.