论文部分内容阅读
研究幽门螺杆菌（Hp）感染对胃粘膜上皮细胞凋亡的影响及其在胃癌发生中的意义。采用脱氧核糖核酸末端转移酶介导的缺口末端标记（TUNEL）技术对Hp阴性患者、Hp阳性患者Hp清除前后胃粘膜上皮中凋亡细胞的分布和密度进行原位观察和比较。结果：发现Hp阳性患者胃粘膜上皮细胞凋亡指数（16.01％），明显高于Hp阴性患者（3．52％）（P＜0．01）；胃粘膜细胞凋亡指数与Hp感染程度明显相关（P＜0．05）；Hp清除后胃粘膜上皮细胞凋亡指数（4.19％）较治疗前明显降低（P＜0．01），而持续阳性者凋亡指数无明显降低。提示：Hp感染可诱导胃粘膜上皮细胞凋亡，这可能是Hp参与胃癌发生的重要机制之一。
To study the effect of Helicobacter pylori (Hp) infection on gastric mucosal epithelial cell apoptosis and its significance in gastric carcinogenesis. The distribution and density of apoptotic cells in gastric mucosal epithelium before and after Hp eradication in Hp-negative patients and Hp-positive patients were observed and compared in situ using DNA-TdT-mediated nick end labeling (TUNEL). Results: The apoptosis index of gastric mucosal epithelium in Hp positive patients (16.01%) was significantly higher than that in Hp negative patients (3.52%) (P <0.01). The apoptosis index of gastric mucosa was significantly correlated with the degree of Hp infection (P <0.05). The apoptotic index of gastric mucosal epithelium (4.19%) after Hp removal was significantly lower than that before treatment (P <0.01), while the apoptotic index of persistent gastric mucosa was not significantly decreased. Hp infection can induce apoptosis of gastric mucosal epithelial cells, which may be one of the important mechanisms of Hp involved in gastric carcinogenesis.