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AIM:To investigate the prognostic value of vascularendothelial growth factor messenger RNA (VEGF mRNA) inthe peripheral blood (PB) of patients with hepatocellularcarcinoma (HCC) undergoing curative resection.METHODS:Using a reverse-transcription polymerase chainreaction (RT-PCR)-based assay,VEGF mRNA in the PB wasdetermined prospectively in 50 controls and in 50 consecutivepatients undergoing curative resection for HCC.RESULTS:Among the isoforms of VEGF mRNA,VEGF_(165)and VEGF_(121) were expressed.By multivariate analysis,ahigher level of VEGF_(165) in preoperative PB correlated with arisk of HCC recurrence with borderline significance (P=0.050)and significantly with recurrence-related mortality (P=0.048);while VEGF_(121) did not.Other significant predictors of HCCrecurrence included cellular dedifferentiation (P=0.033),an absent or incomplete capsule (P=0.020),vascularpermeation (P=0.018),and daughter nodules (P=0.006).The other significant parameter of recurrence relatedmortality was cellular dedifferentiation (P=0.053).The levelof circulating VEGF mRNA,however,did not significantlycorrelate with tumor size,cellular differentiation,capsule,daughter nodules,vascular permeation,necrosis andhemorrhage of tumors.CONCLUSION:The preoperative level of circulating VEGFmRNA,especially isoform VEGF_(165),plays a significant role inthe prediction of postoperative recurrence of HCC.
To investigate the prognostic value of vascularendothelial growth factor messenger RNA (VEGF mRNA) inthe peripheral blood (PB) of patients with hepatocellularcarcinoma (HCC) undergoing curative resection. METHODS: Using a reverse-transcription polymerase chain reaction (RT-PCR) -based assay, VEGF mRNA in the PB was determined prospectively in 50 controls and in 50 consecutivepatients undergoing curative resection for HCC. RESULTS: Among the isoforms of VEGF mRNA, VEGF_ (165) and VEGF_ (121) were expressed.By multivariate analysis, ahigher level of VEGF_ (165) in preoperative PB correlated with arisk of HCC recurrence with borderline significance (P = 0.050) and significantly with recurrence-related mortality (P = 0.048); while VEGF_ (121) did not.Other significant predictors of HCC recurrence included cellular dedifferentiation (P = 0.033), an absent or incomplete capsule (P = 0.020), vascular hypermeation (P = 0.018), and daughter nodules The level of circulating VEGF mRNA, however, did not significantlycorrelate with tumor size, cellular differentiation, capsule, daughter nodules, vascular permeation, necrosis andhemorrhage of tumors. CONCLUSION: The preoperative level of circulating VEGF mRNA, especially isoform VEGF_ (165), plays a significant role inthe prediction of postoperative recurrence of HCC.