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目的监测北京市通州区手足口病病原的情况。方法 2008～2010年采集患儿、健康儿童和患儿家长的咽拭子、肛拭子等标本,采用逆转录聚合酶链反应(RT-PCR)方法,进行肠道病毒71型(EV71)、柯萨奇病毒A16型(Cox-A16)和其他肠道病毒的核酸检测。结果调查了262例患儿、29名健康儿童和9名患儿家长。患儿中EV71阳性率为25.4%,CoxA16为8.3%,其他肠道病毒为14.9%,各年病毒型别分布差异有统计学意义(P﹤0.05)。48例重症患儿中EV71阳性率为18.8%,其他肠道病毒为12.5%,未检出CoxA16。8名健康儿童其他肠道病毒阳性率为12.8%。患儿家长未检出病毒。结论患儿病毒检出率由高到低分别是EV71、其他肠道病毒和CoxA16。重症患儿中以EV71、其他肠道病毒为主。健康儿童也会携带其他肠道病毒。
Objective To monitor the pathogens of HFMD in Tongzhou District of Beijing. Methods From 2008 to 2010, throat swabs and anal swabs from children, healthy children and their parents were collected. The expression of EV71 and EV71 was detected by RT-PCR, Coxsackie virus A16 (Cox-A16) and other enterovirus nucleic acid detection. Results A total of 262 children, 29 healthy children and 9 parents were surveyed. The positive rate of EV71 in children was 25.4%, that of CoxA16 was 8.3%, and that of other enteroviruses was 14.9%. The distribution of virus types in each year was statistically significant (P <0.05). The positive rate of EV71 was 18.8% in 48 severe cases and 12.5% in other enteroviruses. The positive rate of other enteroviruses in C.81 healthy children was 12.8%. Parents have not detected the virus in children. Conclusions The detection rate of children with virus from high to low is EV71, other enterovirus and CoxA16 respectively. Severe cases of children with EV71, other enterovirus-based. Healthy children also carry other enteroviruses.