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目的 建立RT PCR检测胃周淋巴结胃癌转移细胞的方法 ,评价其临床应用价值。方法 以MUC1cDNA的引物建立了RT PCR扩增体系及PAGE 银染检测PCR扩增产物的方法 ,并作了优化 ;在对扩增体系的检测特异性和敏感性作了分析后 ,初步检测了临床样品。结果 该扩增体系具有较好的特异性 ,敏感性可达 10 - 6 μgRNA ,相当于从 10 5个淋巴细胞中检出 1个胃癌细胞 ;对临床样品检测的结果与病理检查结果相符。结论 该法具有较好的可靠性 ,可作为临床常规病理探查的补充检查手段
Objective To establish a RT PCR method for the detection of lymph node metastasis in gastric cancer and evaluate its clinical value. METHODS: The PCR amplification system of RT PCR and the PCR amplification product of PAGE silver staining were established with the primers of MUC1 cDNA and optimized. After analyzing the detection specificity and sensitivity of the amplification system, the clinical detection was performed. sample. RESULTS: The amplified system had good specificity with a sensitivity of 10 -6 μg RNA, which was equivalent to detecting one gastric cancer cell from 10 5 lymphocytes. The results of clinical samples were consistent with the results of pathological examination. Conclusion This method has good reliability and can be used as a supplementary examination method for clinical routine pathological exploration.