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目的研究脑源性神经营养因子(BDNF)及酪氨酸蛋白激酶受体B(TrkB)在舌鳞状细胞癌(TSCC)中的表达情况,并观察外源性BDNF对舌鳞状细胞癌增殖活性的影响。方法采用免疫组织化学技术检测BDNF、TrkB在50例TSCC和13例正常舌黏膜中的表达;以舌鳞癌细胞系Tca8113为研究对象,采用免疫荧光技术检测BDNF、TrkB的表达,CCK8细胞增殖实验观察外源性BDNF对舌鳞癌细胞系Tca8113增殖活性的影响。结果BDNF/TrkB在舌鳞状细胞癌组织标本中阳性表达率显著高于正常舌黏膜组织(P<0.05),且两者的表达与浸润深度及淋巴转移有关。高浓度BDNF(200 ng/m L)可显著促进Tca8113的增殖活性(P<0.05)。结论 BDNF/TrkB可能与舌鳞状细胞癌的发生发展密切相关,BDNF可显著提高舌鳞状细胞癌的增殖能力。
Objective To investigate the expression of brain derived neurotrophic factor (BDNF) and tyrosine kinase K (TrkB) in tongue squamous cell carcinoma (TSCC) and to observe the effect of exogenous BDNF on the proliferation of tongue squamous cell carcinoma Effect of activity. Methods The expression of BDNF and TrkB in 50 cases of TSCC and 13 cases of normal tongue mucosa was detected by immunohistochemistry. The expression of BDNF and TrkB in tongue squamous cell carcinoma cell line Tca8113 was detected by immunofluorescence technique. The proliferation of CCK8 cells To observe the effect of exogenous BDNF on the proliferation of tongue squamous cell carcinoma cell line Tca8113. Results The positive rate of BDNF / TrkB in tongue squamous cell carcinoma was significantly higher than that in normal tongue mucosa (P <0.05), and the expression of BDNF / TrkB was correlated with the depth of invasion and lymphatic metastasis. High concentration of BDNF (200 ng / m L) could significantly promote the proliferation of Tca8113 (P <0.05). Conclusion BDNF / TrkB may be closely related to the occurrence and development of tongue squamous cell carcinoma. BDNF can significantly increase the proliferation of tongue squamous cell carcinoma.