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目的:建立舒胸口腔崩解片中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法:采用高效液相色谱法,Kromasil-C18色谱柱(4.6mm×250mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速1.0mL·min-1,柱温30℃,检测波长203nm。结果:三七皂苷R1、人参皂苷Rg1和Rb1的线性范围分别为0.41~4.08μg(r=0.9997)、0.80~8.04μg(r=0.9993)、0.82~8.20μg(r=0.9994)。该方法平均回收率三七皂苷R1为98.97%(RSD=2.25%,n=6)、人参皂苷Rg1为101.98%(RSD=2.55%,n=6)、人参皂苷Rb1为98.71%(RSD=2.91%,n=6)。结论:该法简便、准确、重现性好,可用于舒胸口腔崩解片中3种皂苷的含量测定。
Objective: To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Shu chest orally disintegrating tablets. Methods: High performance liquid chromatography (HPLC) was performed on a Kromasil-C18 column (4.6 mm × 250 mm, 5 μm) using gradient elution with acetonitrile-water as mobile phase at a flow rate of 1.0 mL · min- 203nm. Results: The linear ranges of notoginsenoside R1, ginsenoside Rg1 and Rb1 were 0.41 ~ 4.08μg (r = 0.9997), 0.80 ~ 8.04μg (r = 0.9993) and 0.82 ~ 8.20μg (r = 0.9994), respectively. The average recovery of this method was 98.97% (RSD = 2.25%, n = 6), ginsenoside Rg1 was 101.98% (RSD = 2.55%, n = 6) and ginsenoside Rb1 was 98.71% %, n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used to determine the content of three saponins in Shu chest orally disintegrating tablets.