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内含子在转基因动物的基因表达中具有重要作用，以乳清酸蛋白（ＷＡＰ）基因５′区为调控序列，人基因组Ｇ－ＣＳＦ基因为目的片段，将ＷＡＰ基因第一内含子插入Ｇ－ＣＳＦ基因５′端，构建成转基因动物乳腺表达载体。将其直接注射到小鼠乳腺，在泌乳期表达出人Ｇ－ＣＳＦ。表明内含子在５′端的位置不影响转基因的表达，同时也表明内含子对表达有一定的作用。
Intron plays an important role in the gene expression of transgenic animals. Using the 5 ’region of the whey protein (WAP) gene as the regulatory sequence and the human genome G-CSF gene as the target fragment, the first intron of the WAP gene is inserted into the G -CSF gene 5 ’end, constructed into a transgenic animal mammary gland expression vector. They were injected directly into mouse mammary glands and expressed human G-CSF during lactation. The results showed that the 5 ’end of the intron did not affect the expression of the transgene, but also showed that the intron had a certain effect on the expression.