以我国普通野生稻基因组 DNA为模板 ,以特异引物经聚合酶链式反应方法扩增出凝集素基因并克隆到 E.coli质粒 p Bluescript SK( +)的 Sma I位点。序列分析表明 ,克隆到的基因片段大小为 781bp,没有内含子 ,编码一条长 2 2 7个氨基酸、分子量约 2 3k D的肽链 ,其中 N-端 2 8个氨基酸是信号肽。与报道的栽培稻凝集素 c DNA序列进行顺序同源性比较 ,发现他们之间有很高的同源性 ( 99.48% ) ,其非编码区第 32位碱基缺失 ,编码区第 163、189和 5 85位各有一个碱基突变 ,但三者均为同义突变 ,并未造成氨基酸序列的变化。鉴于凝集素可能具有抗病、虫害的能力 ,普通野生稻凝集素基因的克隆及序列分析有助于进一步揭示水稻凝集素在植物防御以及其它生理活动中的功能 ,并为植物抗病虫基因工程提供有用的素材 The genomic DNA of common wild rice (Oryza sativa) from China was used as a template to amplify the lectin gene by polymerase chain reaction (PCR) with specific primers and cloned into the Sma I site of E. coli plasmid p Bluescript SK (+). Sequence analysis showed that the cloned gene fragment was 781 bp in length without introns and encoded a peptide chain with a length of 277 amino acids and a molecular weight of about 23 kD. The 28 amino acids at the N-terminal end were signal peptides. Compared with sequence homology of reported lectin c DNA sequence of the cultivated rice, it was found that there was a high homology (99.48%) among them. The 32nd base of its non-coding region was deleted. The coding region 163,189 And 8585 each have a base mutation, but all three are synonymous mutations, did not cause changes in amino acid sequence. In view of the possible resistance of lectins to diseases and pests, the cloning and sequence analysis of Lectin gene of common wild rice will help to further reveal the function of Lectins in plant defense and other physiological activities, Provide useful material