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目的了解2012年广西壮族自治区(广西)罗城县诺如病毒流行株基因型谱分布。方法 2012年收集323例5岁以下腹泻患儿粪便标本,反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测诺如病毒,对VP1区部分核苷酸序列进行测定并构建核苷酸系统进化树。结果检测323例病例粪便标本,诺如病毒阳性24份(7.43%);诺如病毒GⅠ基因组2份(8.33%),GⅠ.4和GⅠ.6各1份;GⅡ基因组22份(91.67%),GⅡ.6基因型1份(1/22),GⅡ.4基因型21份(21/22)。GⅡ.4中,18份(85.71%)属于2006b变异株簇,3份(14.29%)属于Sydney变异株簇。结论 2012年广西罗城县诺如病毒流行株基因型呈现多样性,以GⅡ.42006b变异株为优势流行株,首次检出GⅡ.4 Sydney变异株。
Objective To understand the genotypic distribution of Norovirus epidemic strains in Luocheng County of Guangxi Zhuang Autonomous Region (Guangxi Province) in 2012. Methods A total of 323 stool specimens from children with diarrhea under 5 years of age were collected in 2012. Norovirus was detected by reverse transcription-polymerase chain reaction (RT-PCR) and the nucleotide sequence of VP1 was determined And construct the nucleotide phylogenetic tree. RESULTS: Staphylococcus aureus samples from 323 stool samples were positive for Norovirus (24.43%), 2 copies of Norovirus (8.33%), 1 copy of GⅠ.4 and GⅠ.6, 22 copies (91.67%) of GⅡ, 1 genotype (1/22) of GⅡ.6 genotype and 21 genotypes (21/22) genotype GⅡ.4. In GⅡ.4, 18 (85.71%) belonged to the 2006b mutant and 3 (14.29%) belonged to the Sydney mutant. Conclusion The genotypes of Norovirus epidemic strains in Luocheng County of Guangxi Province showed diversity in 2012. The G Ⅱ .4 Sydney variant was detected for the first time with G Ⅱ .42006b mutant as the predominant epidemic strain.