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目的探究川穹嗪对甲醛所致炎性痛小鼠脊髓背角ERK表达变化的影响,以评价川穹嗪对炎性痛的镇痛效果。方法选取昆明小鼠30只,体重约30g,不分雌雄,常规条件饲养。随机分为正常组(n=6),生理盐水预处理组(n=12)及川穹嗪(TMP)药物干预组(n=12)。TMP预处理组在注射甲醛之前灌胃TMP(20g/L),按小鼠体重0.05ml/10g灌胃30d。生理盐水组小鼠灌胃等量生理盐水。两组均用微量注射器注射0.02ml 4%甲醛到小鼠右侧脚掌皮下从而建立炎性疼痛模型,通过观察小鼠舔足和咬足时间进行行为学检测,以及免疫组化荧光检测脊髓背角ERK变化。结果在疼痛第一时相(注射PF后10min内),NS预处理组与TMP预处理组两者之间行为学检测没有统计学差异;在疼痛第一时相(注射PF后10~45min),小鼠NS预处理组注射PF后,舔足咬足持续时间可达285s,TMP预处理组注射PF后,舔足咬足持续时间可达145s,两者相比有统计学差异(P=0.0072)。实验小鼠脊髓背角ERK免疫组化荧光检测,TMP预处理组与生理盐水预处理组相应时间点相比,TMP预处理组PF给药30min、60min亚组小鼠脊髓背角ERK阳性表达均弱于前者,有统计学意义(P<0.05)。结论TMP能调节小鼠背角ERK表达从而调节PF致炎性痛的程度。
Objective To investigate the effect of Chuan Qunzine on the expression of ERK in the spinal dorsal horn induced by formaldehyde-induced inflammatory pain in mice to evaluate the analgesic effect of Chuan Qiongzheng on inflammatory pain. Methods Kunming mice 30, weighing about 30g, regardless of male and female, conventional conditions breeding. The rats were randomly divided into normal group (n = 6), saline pretreatment group (n = 12) and TMP drug intervention group (n = 12). The TMP pretreatment group was given TMP (20 g / L) before the injection of formaldehyde, and the mice were gavaged with 0.05 ml / 10 g body weight for 30 days. The mice in normal saline group were given the same amount of normal saline. Both groups were injected with 0.02ml 4% formaldehyde to the right foot of the mouse subcutaneous so as to establish a model of inflammatory pain by microinjection syringe, behavioral tests were performed by observing the licking of foot and bite time, and immunohistochemical staining for spinal dorsal horn ERK changes. Results In the first phase of pain (within 10 min after injection of PF), there was no significant difference in behavior between NS pretreatment group and TMP pretreatment group. In the first phase of pain (10 ~ 45 min after PF injection) , And the duration of bite in licking foot was up to 285s after PF was injected into NS pretreatment group. The duration of licking foot bite in TMP pretreatment group was 145s after PF injection (P = 0.0072). Compared with the corresponding time point in the saline preconditioning group, the expression of ERK in the spinal cord dorsal horn of TMP pretreatment group for 30min and 60min Weaker than the former, with statistical significance (P <0.05). Conclusion TMP can regulate the expression of ERK in the dorsal horn of mice and thus regulate the degree of inflammatory pain induced by PF.