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目的:探讨肝癌患者肝移植手术前后血清中miR-221的表达变化及其临床意义。方法:选择2009年6月至2011年8月在我院行肝移植手术的42例肝癌患者、26例肝良性疾病患者和40例正常对照组人员为研究对象,采用实时荧光定量RT-PCR(Real-time RT-PCR)对比检测移植前后患者血清中miR-221的表达水平。根据肝移植术前患者血清miR-221表达水平的平均值作为划分标准,将36例患者分为高表达组和低表达组。分析miR-221的表达与移植术后肿瘤复发和转移的关系。结果:原发性肝癌患者血清miR-221表达水平明显高于正常对照组(P<0.05)。移植术后患者血清miR-221的表达显著降低(P<0.01)。术前血清miR-221水平与肝移植术后HCC转移/复发情况密切相关。高表达组肝移植患者术后肝癌转移/复发率明显高于低表达组患者(P<0.05);术后6月内肝癌复发患者血清miR-221水平显著高于未复发者(P<0.05)。结论:血清miR-221表达水平与肝癌肝移植术后肿瘤复发和转移密切相关,有可能成为肝癌肝移植预后的评估指标。
Objective: To investigate the changes of serum miR-221 level in liver cancer patients before and after liver transplantation and its clinical significance. Methods: Forty-two patients with liver cancer who underwent liver transplantation in our hospital from June 2009 to August 2011 were enrolled. Twenty-six patients with benign liver disease and 40 normal control subjects were selected as experimental subjects. Real-time fluorescent quantitative RT-PCR Real-time RT-PCR was used to detect the level of miR-221 in serum of patients before and after transplantation. According to the average value of serum miR-221 expression level before liver transplantation, 36 patients were divided into high expression group and low expression group. The relationship between miR-221 expression and tumor recurrence and metastasis after transplantation was analyzed. Results: The serum level of miR-221 in patients with primary liver cancer was significantly higher than that in the normal control group (P <0.05). After transplantation, the expression of miR-221 was significantly decreased (P <0.01). Preoperative serum miR-221 levels are closely related to HCC metastasis / recurrence after liver transplantation. The level of miR-221 in liver cancer recurrence patients was significantly higher than that in non-recurrence patients at 6 months after operation (P <0.05) . Conclusion: The expression level of serum miR-221 is closely related to the recurrence and metastasis of liver cancer after liver transplantation, which may become the prognostic indicator of liver transplantation.