论文部分内容阅读
目的:探索三七总皂苷注射液的超滤工艺。方法:采用HPLC测定人参皂苷Rg1,Rb1及三七皂苷R1的含量,通过单因素试验考察不同相对分子量超滤膜、药液质量浓度、温度、洗液体积对三七总皂苷注射液超滤工艺的影响。结果:确定的超滤工艺为三七总皂苷水溶液质量浓度100 g.L-1,选用10 kDa超滤膜,超滤温度70℃,洗液体积1 200 mL,人参皂苷Rg1,Rb1及三七皂苷R1各组份透过率均>90%。结论:优选的超滤工艺用于三七总皂苷注射液的纯化是可行的。
Objective: To explore the Panax notoginseng saponin injection ultrafiltration process. Methods: The contents of ginsenosides Rg1, Rb1 and notoginsenoside R1 were determined by HPLC. The single factor experiments were used to investigate the effects of different molecular weight ultrafiltration membranes, liquid mass concentration, temperature and volume of washing liquid on ultrafiltration Impact. Results: The determined ultrafiltration process was 100 gL-1 of Panax notoginseng saponin aqueous solution, 10 kDa ultrafiltration membrane, ultrafiltration temperature 70 ℃, volume of washing liquid 1 200 mL, ginsenoside Rg1, Rb1 and notoginsenoside R1 The transmittance of each component is> 90%. Conclusion: The optimal ultrafiltration process is feasible for the purification of Panax notoginseng saponin injection.