【摘 要】
：
Two haptens of 3-[(5-amino-furan-2-ylmethylene)amino]oxazolidin-5-one(FZ-NH2)and 3-{[(4-carboxypbenyl)methylene]-amino}-2-oxazolidinone(CPAOZ)were synthesized.F
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Two haptens of 3-[(5-amino-furan-2-ylmethylene)amino]oxazolidin-5-one(FZ-NH2)and 3-{[(4-carboxypbenyl)methylene]-amino}-2-oxazolidinone(CPAOZ)were synthesized.For FZ-NH2,immunogens were prepared by glutaraldehyde and diazo salt methods.For CPAOZ,immunogens were connected by the methods of the active ester and mixed acid anhydride.Compared with the combination,indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was developed with coating antigen of FZ-NH2-OVA via the glutaraldehyde method and immunogen of CPAOZ-KLH via active ester method.For furazolidone and its metabolite AOZ(NPAOZ as derivative),the sensitivities(IC50)were 2.0 μg/L and 2.5 μg/L,limits of detection(IC15)were0.09 μg/L and 0.25 μg/L,respectively.A sensitive method was developed for the simultaneous determination of furazolidone in feed and its metabolite AOZ in tissue.
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