论文部分内容阅读
灭菌土用利福霉素抗性细菌(Ecc RL4)接种后,播种大白菜,幼芽的成苗率降低,且表现矮化和叶色变深的症状。用含利福霉素的改良肉汁胨培养基分离,可以从病苗中回收到病原细菌。组织中病原细菌的含量为10~2—10~7CFU/克不等。病菌可从萌发过程中的芽和整个幼苗期的根部侵入,侵入后,从整个生育期的幼苗直到收获后的菜株内均可回收到病原菌。不同器官的含菌量有显著差别,通常以茎与叶柄的含菌量为高,叶片以半功能叶含菌量为高,根表现不稳定。幼苗带菌率50—100%不等,且随生育期的延长而增高,在播种后第17天均达100%。检测带菌率和腐烂率时,采用了厌气富集技术和含利福霉素的培养基,大大提高了组织内病原细菌的检出率和分离的选择性。
After inoculation with rifamycin-resistant bacteria (Ecc RL4) in sterilized soil, the seedlings of Chinese cabbage were sown and the germination rate of shoots decreased, and the symptoms of dwarfing and darkening of leaf color were observed. Pathogenic bacteria can be recovered from the vaccine using rifampicin modified broth culture medium. The content of pathogenic bacteria in tissues is 10 ~ 2-10 ~ 7CFU / g. Bacteria can invade the buds during germination and the root of the entire seedling stage. After invade, the pathogen can be recovered from the seedlings throughout the growing season up to the harvested plant. Different organs have significant differences in the amount of bacteria, usually containing high amounts of bacteria in the stem and petiole, leaf half-functional leaves with high bacterial content, root instability. Seedling rates ranged from 50% to 100%, and increased with the growing period, reaching 100% on the 17th day after sowing. Detection of carrier rate and decay rate, the use of anaerobic enrichment technology and rifamycin-containing medium, greatly increased the detection of pathogenic bacteria in the organization and the selectivity of separation.