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首次从长春花中克隆了Crlea(Crlea for Catharanthus roseuslate embryogenesis abundant)的全长基因,采用荧光定量PCR方法对干旱胁迫下长春花叶片和根部Crlea基因的表达模式进行监测,结果表明,在0·5~8h的胁迫时间中,叶片和根部的Crlea基因表现出相似的积累模式。长春花Crlea基因的表达随着胁迫时间的延长而表达增强。在叶片中,在6h和8h的干旱处理后,Crlea基因表达显著提高,分别是未处理材料的9·984和20·431倍。在根部,在8h的处理后,Crlea基因的表达量显著提高(2·831倍于对照)。初步结果表明Crlea基因的表达没有组织特异性,并且为干旱胁迫正调控。
The full-length gene of Crlea (Crlea for Catharanthus roseuslate embryogenesis abundant) was cloned for the first time. Fluorescence quantitative PCR was used to monitor the expression pattern of Crlea gene in leaves and roots under drought stress. The results showed that at 0.5 During the stress time of ~ 8h, the Crlea gene in leaves and roots showed a similar pattern of accumulation. The expression of Crlea gene of Catharanthus rosea increased with the extension of stress time. In the leaves, Crlea gene expression was significantly increased after 6 h and 8 h of drought treatment, which were 9.94 and 20.431 times that of the untreated material, respectively. At the root, Crlea gene expression increased significantly (831 times the control) after 8 h of treatment. Preliminary results indicate that Crlea gene expression is not tissue-specific and is positively regulated by drought stress.