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一些研究间接证明,过氧化损伤与老年神经系统疾病(如帕金森病和阿尔茨海默病)有关。随龄增加的过氧化物可破坏神经细胞膜完整性,修饰震颤素,刺激乙酰胆碱分泌,改变膜G蛋白信息传递,使黑质纹状体环路的多巴胺(DA)释放减少。膜胆固醇/磷脂比值的随龄增加及膜胆固醇(CHO)的增加和氧化修饰均能促进膜损伤。为此,作者用CHO孵育纹状体切片然后暴露于H2O2,并观察相应指标变化以进一步证实上述推论。材料和方法纹状体组织切片取自344只成龄(6个月)和老龄(24个月)鼠,在BRM(basel releasemedium)内孵育并冲洗后分别置5种条件下孵育:单独BRM(对照组)、CHO、H2O2、CHO—H2O2(CHO孵育后暴露于H2O2)、H2O2-CHO(H2O2孵育后再CHO孵育)。采用HPLC双电化学探测法分析DA释放量及硝普钠——胆固醇(NO—CHO)反
Some studies indirectly show that peroxidative damage is associated with senile neurological diseases such as Parkinson’s disease and Alzheimer’s disease. Peroxides with age can disrupt the integrity of the neuronal membrane, modify the tremor hormone, stimulate the secretion of acetylcholine, change the G-protein signaling, and decrease the release of dopamine in the nigrostriatal loop. Membrane cholesterol / phospholipid ratio increased with age and membrane cholesterol (CHO) increased and oxidative modification can promote membrane damage. For this reason, the authors incubated striatum slices with CHO and then exposed to H2O2, and observed changes in the corresponding indicators to further confirm the above reasoning. MATERIALS AND METHODS Striatal tissue sections from 344 adult (6 months old) and aged (24 months old) mice were incubated in BRM (Basel release medium) and rinsed and then incubated under five different conditions: BRM alone CHO, H2O2, CHO-H2O2 (CHO exposed to H2O2 after incubation) and H2O2-CHO (incubated with H2O2 followed by CHO). The release of DA and the nitric oxide-cholesterol (NO-CHO)