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目的:研究藻蓝蛋白对脑缺血再灌注后超氧化物歧化酶(SOD)表达和神经细胞凋亡的影响。方法:成年健康雄性Wistar大鼠52只,应用线栓法经左侧颈外-内动脉插线建立大脑中动脉闭塞再灌注(MCAO/R)模型。应用藻蓝蛋白进行治疗,TUNEL法检测脑缺血再灌注后神经细胞凋亡,免疫组织化学技术检测SOD的表达。结果:脑缺血再灌注后皮质区和纹状体区神经细胞凋亡数量增加,至再灌注1d达高峰;同时SOD于再灌注12h￣7d持续高表达,再灌注1d达高峰。应用藻蓝蛋白后,缺血区神经细胞形态结构未见明显改变,但凋亡细胞数量于再灌注12h￣3d显著减少,同时SOD表达再灌注6h￣3d明显高于对照组,SOD的表达增强时间与细胞凋亡减少的时间相符合,两者成反向变化关系。结论:藻蓝蛋白可能通过激活内源性抗氧化酶SOD的表达而抑制神经细胞凋亡,发挥神经保护作用。
Objective: To study the effects of phycocyanin on superoxide dismutase (SOD) expression and neuronal apoptosis after cerebral ischemia-reperfusion. Methods: Fifty - two adult male Wistar rats were randomly divided into two groups: middle cerebral artery occlusion and reperfusion (MCAO / R) model. The application of phycocyanin for treatment, TUNEL method for detecting neuronal apoptosis after cerebral ischemia-reperfusion, immunohistochemical detection of SOD expression. Results: The number of apoptotic neurons in cortex and striatum increased after cerebral ischemia and reperfusion, and peaked on the 1st day of reperfusion. At the same time, SOD was continuously expressed 12h ~ 7d after reperfusion and peaked on the 1st day after reperfusion. After the application of phycocyanin, the morphological changes of ischemic neuronal cells showed no significant changes, but the number of apoptotic cells decreased significantly 12h ~ 3d after reperfusion, and the expression of SOD increased 6h ~ 3d after reperfusion The time is consistent with the reduction of apoptosis, and the two are inversely related. CONCLUSION: Phycocyanin may play a neuroprotective role by inhibiting the expression of endogenous antioxidant enzyme SOD and inhibiting neuronal apoptosis.