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电离辐射造成的损伤可引起机体对感染易感性的增高。本实验用体内菌落形成单位法(colony forming-units method in vivo,体内CFU法)对空肠弯曲菌CJ-S131株在受照小鼠体内的粘附、定居进行了动态观察,旨在研究辐射对感染易感性影响的机理。实验结果提示:(1)小鼠受不同剂量~(60)Coγ射线全身照射后均出现空弯菌在体内的粘附、定居增加。(2)小鼠受低于10Gy照射时,仅在粘附高峰时粘附指数(adhesive index,Al)显著高于对照组;在15Gy时,从感染后6～48h均可见AI的显著增加;而20Gy照射组,30min时AI即明显增加,并持续整个实验过程。(3)小鼠受照剂量越大,增加的AI亦越大。(4)方差分析提示,增加的AI在各肠段及各感染时间段间均无差别。小鼠受全身照射后对感染的易感性增加,表现为空弯菌在整个肠道粘膜的粘附增加,而无肠段间的区别,并随照射剂量的增大而增加。受照射后肠道局部微环境及肠粘膜表面结构的改变可能对细菌的粘附,定居起促进作用。
Damage caused by ionizing radiation can cause the body’s susceptibility to infection increased. In this study, colony forming-units method (in vivo CFU method) of CJ-S131 Campylobacter jejuni in irradiated mice in vivo adhesion and settlement were observed dynamically to study the radiation pair The mechanism of infection susceptibility. The experimental results suggest that: (1) The adhesion and the colonization of Beauveria bassiana in vivo in mice after whole body irradiation with different doses of ~ (60) Co γ-rays. (2) When the mice were exposed to less than 10Gy, the adhesive index (Al) was significantly higher than that of the control group only at the peak of adhesion. At 15Gy, the AI was significantly increased from 6 to 48h after infection. The 20Gy irradiation group, AI 30min significantly increased, and continued the entire experimental process. (3) The larger the irradiated dose of mice, the greater the increased AI. (4) Analysis of variance showed that the increased AI was not different between the various bowel stages and each infection time. The susceptibility of mice to infection after systemic irradiation was increased, showing an increase in the adhesion of the airy fungi throughout the gut mucosa, without any difference in bowel segments, and increased with increasing irradiation dose. After irradiation, the local intestinal microenvironment and intestinal mucosal surface structure changes may promote the adhesion of bacteria to promote.