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目的:制备硫酸酯化葡甘聚糖-海藻酸钡(SGA)微囊,包裹大鼠胰岛,对比观测SGA微囊的体外生物相容性。方法:溶液共混法制备硫酸酯化葡甘聚糖-海藻酸盐混合凝胶液,改良Omer法制作SGA微囊。X射线衍射分析SGA二元共混膜结构;SGA微囊化SD大鼠胰岛,体外培养14d后,胰岛素刺激释放实验对比SGA微囊和APA、AB(a海藻酸钡)微囊对大鼠胰岛的影响。结果:衍射图谱显示硫酸酯化葡甘聚糖-海藻酸钡共混膜在原属于海藻酸钡的2θ11°、20.3°结晶峰变小,在2θ29.8。出现新结晶峰,说明SGA二元共混膜相互作用强烈,融合较好;胰岛素刺激释放实验显示,体外培养后SGA微囊对大鼠胰岛的影响与APA、ABa微囊无差别(P>0.05)。结论:SGA微囊膜结构稳定,融合度较好,对大鼠胰岛体外生物相容性较高,可以为下一步体内移植所应用。
OBJECTIVE: To prepare the sulfated glucomannan-alginate (SGA) microcapsules and encapsulate rat islets, and to observe the in vitro biocompatibility of SGA microcapsules. Methods: Sulfated glucomannan - alginate gel solution was prepared by solution blending method and modified Omer method was used to make SGA microcapsules. X-ray diffraction analysis SGA binary blend membrane structure; SGA microencapsulated SD rat islets, in vitro 14 days after stimulation of insulin release experiments SGA microcapsules and APA, AB (a barium alginate) microcapsules on rat islets Impact. RESULTS: The diffraction pattern showed that the sulfate esterified glucomannan-barium alginate film had a smaller peak at 2θ11 ° and 20.3 °, at 2θ29.8. The results showed that the interaction of SGA binary blend membrane was strong and the fusion was good. Insulin stimulation release experiment showed that the effect of SGA microcapsules on rat islets had no difference with APA and ABa microcapsules (P> 0.05 ). CONCLUSIONS: SGA microcapsules have stable membrane structure and good confluence, and have high biocompatibility in vitro to rat islets for further application in vivo.