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阐释荚果相关性状的遗传基础是改良花生品种产量的重要前提。本研究以高产高油新品种中花16及其2个直接亲本和6个间接亲本为材料,对荚果性状及其相应SSR标记进行鉴定。结果表明,中花16的荚果长、百果重和百仁重低于其直接亲本8130和中花5号,但高于全部供试8个亲本的平均值;荚果宽和种子宽大于直接亲本和间接亲本的对应值;种子长高于直接亲本和8个亲本的平均值。通过136对多态性SSR引物鉴定表明,中花16具有直接亲本8130的64.1%和中花5号87.3%的条带,且中花16还具有一些直接亲本中不存在而在间接亲本中存在的一些条带。综合分析SSR标记带型和表型数据,鉴定出5个与百果重相关、4个与百仁重相关、3个与荚果长相关、3个与种子长相关的候选分子标记。综合分析表明,直接和间接亲本中与大果性状相关的标记IPAHM606-1、IPAHM531-1、GA8-2、1B9-4和HAS0428-2条带在育种过程中传递给了中花16。
Explaining the genetic basis of pod-related traits is an important prerequisite for improving peanut yield. In this study, Zhonghua 16, its two direct parents and six indirect parents were used as materials to identify pod characters and their corresponding SSR markers. The results showed that the pod length, berry weight and berry weight of Zhonghua 16 were lower than their direct parents 8130 and Zhonghua 5, but higher than the average of all 8 parents tested. The pod width and seed width were greater than those of the direct parents And the corresponding value of the indirect parent; the seed length is higher than the average of the direct parent and the eight parents. Identification of 136 polymorphic SSR primers showed that Zhonghua 16 had 64.1% of the direct parent 8130 and 87.3% of the Zhonghua 5, and Zhonghua 16 also had some direct parents absent in indirect parents Some stripes. Through comprehensive analysis of SSR marker bands and phenotypic data, five candidate molecular markers were identified that were correlated with the fruit weight, four with 100 kernel weight, three with pod length, and three with seed length. Comprehensive analysis showed that the bands of IPAHM606-1, IPAHM531-1, GA8-2, 1B9-4 and HAS0428-2 related to large fruit traits in direct and indirect parents were transferred to Zhonghua16 during breeding.