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采用还原法制备了AuNPs/MWCNTs复合材料,并构建了氧化还原蛋白质的固定化和生物传感界面AuNPs/MWCNTs/GC电极.以肌红蛋白(Myoglobin,Mb)为例,研究了固定化蛋白质在AuNPs/MWCNTs/GC电极上的直接电化学.结果表明,AuNPs/MWCNTs复合材料不仅能有效地促进Mb与电极表面的直接电子转移,而且能很好地保持固定化Mb的生物催化活性.Mb/AuNPs/MWCNTs/GC电极对H2O2具有良好的电催化还原性能,其线性响应范围为1～138μmol·L-1,检测限为0.32μmol·L-1(S/N=3),并具有较低的米氏常数(0.143 mmol·L-1).该电极操作简单,响应迅速,稳定性和重现性好,有望用于蛋白质的固定化及第三代生物传感器的制备.
The AuNPs / MWCNTs composites were prepared by the reduction method and AuNPs / MWCNTs / GC electrodes were immobilized on the redox proteins and the biosensor interface was constructed.Using Myoglobin (Mb) as an example, AuNPs / MWCNTs / GC electrode.The results show that the AuNPs / MWCNTs composite can not only promote the direct electron transfer between Mb and electrode surface, but also keep the biocatalytic activity of immobilized Mb well.Mb / The AuNPs / MWCNTs / GC electrode has good electrocatalytic reduction performance for H2O2 with a linear response range of 1 ~ 138μmol·L-1 and a detection limit of 0.32μmol·L-1 (S / N = 3) (0.143 mmol·L-1) .The electrode has the advantages of simple operation, rapid response, good stability and reproducibility, and is expected to be used for protein immobilization and preparation of the third-generation biosensor.