论文部分内容阅读
采用ＰＣＲ扩增１６—２３ｓｒＲＮＡ基因间序列和限制性片段长度多态性（ＲＦＬＰ）法，分析了２７株属于快生、慢生二个类型的大豆根瘤菌。慢生大豆根瘤菌都产生了一条约１９０４ｐｂ大小的ＤＮＡ产物带。用ＨａｅⅢ、ＭｓｐⅠ和ＣｏｆⅠ限制性酶处理２７株菌株ＰＣＲ扩增产物，除ＤＨ４４４和ＬＬ１６外，其余菌株３种酶处理的结果一致，可以分成９种遗传模式组。９种遗传模式组与菌株的地理起源相关，但与细菌的血清学分组无直接关系。利用大豆根瘤菌１６—２３ｓｒＲＮＡ基因序列间的多态性特性，采用ＰＣＲ—ＲＦＬＰ法分组，比血清学更能反映根瘤菌的遗传进化关系。
Twenty-seven strains of soybean rhizobia belonging to fast-growing and slow-growing species were analyzed by PCR amplification of 16-23srRNA intergenic sequences and restriction fragment length polymorphism (RFLP). Bradyrhizobium japonicum produces a DNA product band of about 1904 pb in size. 27 strains of PCR amplification products were treated with Hae Ⅲ, Msp Ⅰ and Cof Ⅰ restriction enzymes. Except for DH444 and LL16, the results of three kinds of enzyme treatment of other strains were the same and could be divided into 9 kinds of genetic model groups. The 9 patterns of genetic patterns are related to the geographical origin of the strains but not to the bacterial serological grouping. Based on the polymorphism of Rhizobium japonicum 16-23srRNA gene sequences, the PCR-RFLP method was more effective than serology to reflect the genetic evolution of rhizobia.