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绝大多数组织培养液中含有葡萄糖,因为它是细胞生命活力的主要能源。近来证实,谷氨酰胺也可以作为细胞的能源。特别是当葡萄糖和氧的利用受到限制时,杂交瘤细胞培养液含有高浓度的葡萄糖和谷氨酰胺。
The vast majority of tissue culture broth contains glucose because it is the main source of energy for cell life. Recently confirmed that glutamine can also be used as a cell energy. In particular, when the utilization of glucose and oxygen is limited, the hybridoma cell culture medium contains high concentrations of glucose and glutamine.