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利用现场三维全内反射荧光光谱法研究了细胞色素Ｃ在不同电极表面的吸附特性，发现与细胞色素Ｃ溶液的荧光峰峰位相比，吸附在裸露银电极表面的细胞色素Ｃ的荧光峰峰位发生蓝移，表明埋在细胞色素Ｃ多肽链内血红素的暴露面积减少，而使细胞色素Ｃ的电化学反应不可逆．当细胞色素Ｃ吸附在促进剂修饰的银电极表面时，其荧光峰峰位基本上不发生蓝移，表明血红素的暴露面积基本上不减少，而能观察到细胞色素Ｃ准可逆的电化学反应．
Three-dimensional total internal reflection fluorescence spectroscopy was used to study the adsorption characteristics of cytochrome C on different electrode surfaces. Compared with the peak of fluorescence peak of cytochrome C solution, the peak of fluorescence peak of cytochrome C adsorbed on the surface of bare silver electrode A blue shift occurred, indicating a reduction in the exposed area of heme buried in the cytochrome C polypeptide chain, leaving the electrochemical reaction of cytochrome C irreversible. When cytochrome C is adsorbed on the surface of accelerator-modified silver electrode, there is almost no blue shift of its fluorescence peak position, indicating that the exposed area of heme is not substantially reduced and the quasi-reversible electrochemical of cytochrome C can be observed reaction.