论文部分内容阅读
目的研究转HJC-1和G6-EPSPS基因大米大鼠主要营养素吸收利用率。方法将140只Wistar大鼠随机分为7组,每组20只,雌雄各半,即转基因大米低、中、高剂量3个组,亲本对照低、中、高剂量3个组和饲养对照组,相应饲料喂养7 d。比较各组大鼠蛋白质功效比值及蛋白质、脂肪、碳水化合物、纤维素的表观消化率等。结果除雄性亲本高剂量组蛋白质功效比值高于饲养对照组外,其他各组蛋白质功效比值与饲养对照组差别无统计学意义;除雌鼠转基因低剂量组的蛋白质表观消化率和雄鼠亲本低剂量组的脂肪表观消化率外,其他各组的蛋白质表观消化率、脂肪表观消化率、碳水化合物表观消化率、纤维素表观消化率均高于饲养对照组(P<0.01);转基因各组与相应亲本对照组上述指标的差异均无统计学意义。结论喂饲转HJC-1和G6-EPSPS基因大米和亲本对照大米的大鼠主要营养素(蛋白质、脂肪、碳水化合物、纤维素)吸收利用率基本具有实质等同性。
Objective To study the main nutrient uptake and utilization of HJC-1 and G6-EPSPS transgenic rice rats. Methods 140 Wistar rats were randomly divided into 7 groups, 20 in each group, male and female were divided into three groups: low, middle and high doses of genetically modified rice. The control groups were low, middle and high dose and the control group , The corresponding feed 7 d. The protein efficiency, protein, fat, carbohydrates and apparent digestibility of cellulose of rats in each group were compared. Results The protein efficiency ratio of high dose group was higher than that of the control group, while the other groups had no significant difference in the protein efficiency ratio between the high dose group and the control group. The apparent digestibility of protein in the low dose transgenic group was lower than that in the male parent The apparent digestibility of protein, apparent digestibility of fat, apparent carbohydrate digestibility, and apparent digestibility of cellulose in the other groups were higher than those in the control group (P <0.01) There was no significant difference in the above indexes between transgenic groups and corresponding parental control group. Conclusion The absorption and utilization of major nutrients (protein, fat, carbohydrates and cellulose) in rats fed with HJC-1 and G6-EPSPS genetically modified rice and their parental control rice are essentially substantively equivalent.