论文部分内容阅读
目的 构建日本血吸虫 RNA聚合酶 转录延长因子 S - p15真核正反义表达载体 ,为进一步鉴定该因子的生物学功能奠定基础。方法 采用表达序列标签法从日本血吸虫成虫 c DNA文库中获得了 1个 RNA聚合酶转录因子 (S - p15 )全长 c DNA序列 ,克隆及序列测定后 ,将该片段正向及反向插入到真核表达载体 pc DNA 3中。重组载体采用氨苄青霉素 L B培养基筛选、双酶切、PCR及测序鉴定。结果 经鉴定 ,S - p15 - pc DNA3真核正反义表达载体构建成功。结论 构建成功 S -p15 - pc DNA3真核正反义表达载体 ,为下一步鉴定其生物学功能奠定基础。
Objective To construct the eukaryotic antisense and antisense expression vector of RNA polymerase elongation factor S - p15 of Schistosoma japonicum, and lay a foundation for further identification of the biological function of this factor. Methods One full - length cDNA sequence of RNA polymerase (S - p15) was obtained from the cDNA library of adult Schistosoma japonicum c DNA by using expression sequence tagging method. After cloned and sequenced, Eukaryotic expression vector pcDNA 3. The recombinant vector was screened by ampicillin LB medium, double enzyme digestion, PCR and sequencing. Results The S - p15 - pcDNA3 eukaryotic expression vector was successfully constructed. Conclusion The successful construction of eukaryotic sense and antisense expression vector of S-p15 - pcDNA3 laid the foundation for the identification of its biological function in the next step.