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本研究采用RACE技术,从巴西橡胶树中鉴定出一个线粒体内膜转位因子TIM23基因。该基因全长c DNA为898 bp,最长开放阅读框567 bp,预测编码蛋白包含188个氨基酸;序列比对分析发现,该基因编码蛋白具有转膜区和PRAT结构域,与拟南芥TIM23-1蛋白具有较高的相似性,将该基因命名为Hb TIM23-1。实时定量RT-PCR分析结果表明,Hb TIM23-1在巴西橡胶树胶乳、叶片、树皮、雄花、雌花、花药中均有表达。在橡胶树叶片不同发育时期,Hb TIM23-1表达存在变化。与健康橡胶树相比,死皮橡胶树胶乳中Hb TIM23-1表达量明显下降。研究发现Hb TIM23-1表达受干旱和低温处理调控,表明HbTIM23-1可能在巴西橡胶树干旱和低温胁迫应答及死皮中发挥作用。
In this study, RACE technique was used to identify a mitochondrial TIM23 translocation in the rubber tree. The full-length cDNA of this gene was 898 bp with the longest open reading frame of 567 bp and the predicted protein contained 188 amino acids. Sequence alignment showed that the gene encoding transmembrane region and PRAT domain interacted with Arabidopsis TIM23 -1 protein has a high similarity, the gene named Hb TIM23-1. The results of real-time quantitative RT-PCR showed that Hb TIM23-1 was expressed in rubber latex, leaves, bark, male flower, female flower and anther. In different developmental stages of rubber tree leaves, Hb TIM23-1 expression changes. Compared with the healthy rubber tree, the expression level of Hb TIM23-1 in dead skin rubber latex decreased significantly. It was found that Hb TIM23-1 expression was regulated by drought and hypothermia, indicating that HbTIM23-1 may play a role in the drought and cold stress response and dead skin of Acacia confusa.