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目的观察灵芝多糖体外对小鼠脾细胞ＩＬ－２、ＩＬ－３ｍＲＮＡ的表达水平是否有影响。方法逆转录多聚酶链式反应（ＲＴ－ＰＣＲ）检测ｍＲＮＡ表达，凝胶图象光密度分析系统进行相对定量。结果在原代小鼠脾细胞培养开始时加入不同浓度的灵芝多糖ＧＬＢ７（５，１０，２０，４０ｍｇ·Ｌ－１），培养１２ｈ及２１ｈ后观察ＧＬＢ７与ＩＬ－２及ＩＬ－３ｍＲＮＡ表达水平之间的量效关系。发现在一定范围内，随着ＧＬＢ７浓度的升高，ＩＬ－２及ＩＬ－３ｍＲＮＡ表达水平也相应提高，对ＩＬ－２ｍＲＮＡ的提高率分别为１０．４％，２１．９％，３７．８％，４６．６％；对ＩＬ－３ｍＲＮＡ的提高率分别为１５．４％，３５．２％，５２．４％，７４．５％。１９９８－０４－０８收稿，１９９８－０７－２６修回＊国家自然科学基金资助课题，Ｎｏ３９４００１６５作者简介：王庆彪，男，２６岁，硕士；雷林生，男，３８岁，医学博士，副教授培养１２ｈ和３０ｈ的上清液中ＩＬ－２样活性及ＩＬ－３样活性处理组与对照组相比亦有明显升高，且有一定的浓度依赖关系。结论灵芝多糖的免疫增强作用与其在转录水平上促进ＩＬ－２及ＩＬ－３ｍＲＮＡ的表达有关
Objective To observe whether Ganoderma lucidum polysaccharides can affect the expression of IL-2 and IL-3 mRNA in mouse spleen cells in vitro. Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression, and relative quantification was performed by gel image optical density analysis system. Results Different concentrations of ganoderma lucidum polysaccharide GLB7 (5, 10, 20 and 40 mg · L-1) were added into the splenocytes of primary mouse at the beginning of culture, and the expressions of GLB7, IL-2 and IL-3 mRNA The dose-effect relationship. Within a certain range, the levels of IL-2 and IL-3 mRNA were also increased with the increase of GLB7 concentration, and the rates of IL-2 mRNA increase were 10.4%, 21.9% and 37.8% , 46.6% respectively. The rates of IL-3 mRNA were 15.4%, 35.2%, 52.4% and 74.5% respectively. 1998-04-08 Received, 1998-07-26 Revised * Supported by the National Natural Science Foundation of China, No39400165 About the author: Wang Qingbiao, male, 26 years old, master; Lei Lingsheng, male, 38 years old, MD, associate professor training IL-2-like activity and IL-3-like activity in supernatant of 12h and 30h also increased significantly compared with the control group, and there was a certain concentration-dependent relationship. Conclusion The immunopotentiation of Ganoderma lucidum polysaccharides is related to the promotion of the expression of IL-2 and IL-3 mRNA at the transcriptional level