目的:探讨乌圆补血口服液对拟老年痴呆症小鼠的预防和治疗效果。方法:实验于2004-06-10/10-03在右江民族医学院重金属与氟砷毒物研究实验室内进行。选用昆明种小白鼠60只,随机分为正常对照组、模型组、乌圆补血口服液预防组、乌圆补血口服液治疗组、脑复康治疗组,每组12只。除正常对照组外,其他各组均用12mg/mL的氯化铝蒸馏水溶液,按303mg/kg灌胃,1次/d,连续115d,制作拟老年痴呆症小鼠模型。乌圆补血口服液预防组从造模开始就用乌圆补血口服液灌服;造模第8周后,乌圆补血口服液治疗组用乌圆补血口服液灌服治疗,脑复康治疗组用脑复康治疗;正常对照组,模型组用等量生理盐水灌胃,1次/d。分别于造模前,造模第8周,治疗后从鼠尾部取血,采用高铁氰化钾法测定各组大鼠血红蛋白,分离血清,采用酶法、改良赖氏法、脲酶-波氏比色法分别测定各组小鼠血清乙酰胆碱酯酶活力、丙氨酸氨基转移酶活力、尿素含量,实验结束后处死各组小鼠,取大脑和肝脏,冰冻下匀浆,用生理盐水制成10%匀浆,测定脑乙酰胆碱酯酶活力、脑丙二醛和肝丙二醛含量(TBA反应法),组间比较采用方差分析,t检验,实验数据以x±s表示,P<0.05有显著性差异。结果:实验纳入小鼠60只,全部进入结果分析。①血红蛋白含量的变化:与造模前比较,造模后第8周乌圆补血口服液预防组及脑复康治疗组的血红蛋白含量明显降低(P<0.01)。治疗后,模型组的血红蛋白含量明显低于造模后第8周(P<0.01),脑复康治疗组在造模后第8周时血红蛋白含量明显低于模型组(P<0.05)。②血清尿素、丙氨酸氨基转移酶、乙酰胆碱酯酶活性活力测定:模型组乙酰胆碱酯酶活性明显高于其他各组[(138.97±16.52)nkat/L,(128.04±12.79,120.46±31.21,117.87±19.45,114.86±16.37)nkat/L,(P<0.05)]。各组小鼠血清尿素含量、丙氨酸氨基转移酶活力各组间无显著性差异(P>0.05)。③脑丙二醛、脑乙酰胆碱酯酶活力和肝丙二醛测定:正常对照组、乌圆补血口服液预防组、乌圆补血口服液治疗组及脑复康治疗组的脑乙酰胆碱酯酶活性明显低于模型组[(12.17±3.17,16.34±15.00,15.00±1.83,14.50±2.00)nkat/L,(23.00±2.67)nkat/L,(P<0.01)]。脑、肝丙二醛含量各组比较无显著性差异(P>0.05)。结论:拟老年痴呆症模型小鼠大脑胆碱能系统受到明显的损害,乌圆补血口服液对其有一定的改善作用。 Objective: To investigate the preventive and therapeutic effects of Wuyuan Buxue Oral Liquid on mice with Alzheimer’s disease. METHODS: The experiment was performed in the Heavy Metals and Fluorine and Arsenic Toxicology Research Laboratory of Youjiang Medical College for Nationalities from June 2004 to March 10, -03. 60 Kunming mice were randomly divided into normal control group, model group, Wuyuan Buxue Oral Liquid Preventive Group, Wuyuan Buxue Oral Liquid Treatment Group, and NaoFuKang Treatment Group, 12 in each group. In addition to the normal control group, the other groups were all treated with a distilled water solution of aluminum chloride (12 mg/mL) at a dose of 303 mg/kg, once daily for 115 days, to prepare a mouse model of Alzheimer’s disease. The Wuyuan Buxue Oral Liquid Prevention Group was fed with Wuyuan Buxue Oral Liquid from the beginning of modeling; after the 8th week of modeling, the Wuyuan Buxue Oral Liquid Treatment Group was treated with Wuyuan Buxue Oral Liquid, and the Naoxifukang Treatment Group. The patients were treated with Nao-Fu-Kang; normal control group and model group were given intragastric administration with equal volume of saline once daily. At the 8th week after modeling, blood was collected from the tail of the rats after the 8th week of modeling, hemoglobin in each group was determined by potassium ferricyanide method, serum was separated, and enzyme method, modified Lai’s method, and urease-Bore ratio were used. The serum acetylcholinesterase activity, alanine aminotransferase activity, and urea level were measured in each group of mice. After the end of the experiment, the mice in each group were sacrificed, and the brain and liver were collected, homogenized under freezing, and made with physiological saline. % homogenate, determination of brain acetylcholinesterase activity, brain malondialdehyde and liver malondialdehyde content (TBA reaction method), the comparison between groups using analysis of variance, t test, experimental data in x ± s, P <0.05 significant Sexual differences. RESULTS: Sixty mice were included in the experiment and all entered the results analysis. 1 Changes in hemoglobin content: Compared with pre-modeling, the hemoglobin content in the Wuyuan Buxue Oral Liquid Prevention Group and the Naofukang Treatment Group decreased significantly (P<0.01) in the 8th week after modeling. After treatment, the hemoglobin content in the model group was significantly lower than the 8th week after model establishment (P<0.01). In the Naofukang treatment group, the hemoglobin content was significantly lower than the model group at the 8th week after modeling (P<0.05). 2 serum urea, alanine aminotransferase, acetylcholinesterase activity assay: model group acetylcholinesterase activity was significantly higher than other groups [(138.97 ± 16.52) nkat / L, (128.04 ± 12.79,120.46 ± 31.21, 117.87 ± 19.45, 114.86 ± 16.37) nkat/L, (P < 0.05)]. There was no significant difference in serum urea content and alanine aminotransferase activity between groups (P>0.05). 3 Brain malondialdehyde, brain acetylcholinesterase activity and hepatic malondialdehyde assay: The activity of brain acetylcholinesterase in normal control group, Wuyuan Buxue Oral Liquid Preventive Group, Wuyuan Buxue Oral Liquid Treatment Group and Naofukang Treatment Group were significantly. Lower than the model group [(12.17±3.17, 16.34±15.00, 15.00±1.83, 14.50±2.00) nkat/L, (23.00±2.67) nkat/L, (P<0.01)]. There was no significant difference in the content of malondialdehyde between brain and liver (P>0.05). Conclusion: The brain cholinergic system of Alzheimer’s disease model mice is obviously damaged, and Wuyuan Buxue Oral Liquid can improve it.